Dr. Gajanand Engla
Devi Ahilya University, Indore, India
Title: In Vitro factory for bioproduction of L-DOPA.
Biography
Biography: Dr. Gajanand Engla
Abstract
Callus culture of Mucuna pruriens (Fabiaceae) were initiated on explants (epicotyls, hypocotyls and cotyledons) obtained from aseptically germinated seedlings on MS media supplemented with NAA (1.0ppm)+BAP (1.0ppm), NAA (0.5ppm)+BAP (0.5ppm), BAP (0.5ppm), BAP (1.0ppm), 2,4-D (1.0ppm)+BAP(0.5ppm) and 2,4-D (2.0ppm)+BAP(1.0ppm). The comparative callus induction efficiency of various explants and hormone combination and concentration used was evaluated, callus cells grew anexically in the presence of various plant growth regulators in Murashige and Skoog’s medium containing 3% w/v of sucrose. The best result was found with epicotyl explants on MS supplemented with. NAA (1.0ppm)+BAP (1.0ppm). The highest callusing was observed with epicotyl explants. Cotyledon explants also showed satisfactory results, while hypocotyl explants has failed to induce callusing on any of explants. The Suspension culture were initiated from epicotyl and cotyledon calli (of NAA/BAP each 1.0ppm & NAA/BAP each 0.5ppm) were initiated and maintained successfully. The callus and cell suspension so obtained, synthesizes various normal constituents of plant out of which, L-DOPA was isolated, identified and estimated. The epicotyl explants showed highest callus induction efficiency i.e. 90%, and cotyledon shows 85%, viability (89% in callus and 74% cell suspension), growth index (3.37) and productivity of L-DOPA (6.62% by Cell DW in callus and 6.58% by cell DW in cell suspension) and it was found higher than the intact Mucuna pruriens seeds (3.61% by cell DW).