5^th World Congress on Natural Products, Medicinal Plants and Traditional Medicines November 05-06, 2018 Singapore City, singapore

Biography:

Paul Toukam has his expertise in promoting human health condition through the development of phytomedicine. His academic training focusing on natural product, the experience gained at the research institute where his is currently employed as permanent staff and the trainings his participated at international level have sharpened his research capability. The number of active principles isolated and identified/characterized from medicinal plants with the use of separating and spectroscopic methods are of great interest for the pharmaceutical industries as well as the local communities whose local concoctions can be improved to traditionally ameliorated medicine as recommended by WHO. If more research projects in phytomedicine are implemented, population of the whole world will be healthier.

Abstract:

Inflammation is a complex process, which is frequently associated with pain and involves occurrences such as: the increase of vascular permeability, increase of protein denaturation and membrane alteration. Inflammation is a leading cause and symptom of much other pathology. According to WHO, osteoarthritic (inflammation of joints and bones) is the cause of 10500 death per year. The management of inflammatory conditions is a real community problem. NSAD are most often used with their adverse side effects. However, alternative treatments are well known by population of developing countries. For example, the stem barks of Pterocarpus erinaceus are used in the form of decoction to treat inflammatory disorders. It is therefore important to promote the discovery and development of new lead compounds. The phytochemical study of the stem bark of Pterocarpus erinaceus led to the isolation of two new compounds a saponin (1) and a benzofuran isoflavonoid (2) along with seven known compounds, friedelin (3),  triacontanoic acid (4), dotriacontanoic acid (5), 2,3-dihydroxypropyl hexacosanoate (6), octacosanoic acid (7), calycosin (8), stigmasterol glucoside (9). On the basis of IR, UV, 1D and 2D NMR analysis in conjunction with EIMS, HRMS and literature review, the structure of the new compounds were characterized. The free radical scavenging activity and serum bovine albumin denaturation activity of the isolated compounds were evaluated. Compound 2 (SC₅₀, 12.63±0.86 μg/mL) and compound 8 (SC₅₀, 42.53±1.77 μg/mL) showed antioxidant property although lesser than that of the reference drug ascorbic acid (SC₅₀, 5.99±0.59 μg/mL). Compound 3 (IC₅₀, 14.87±1.51 μg/mL) was the most active against the denaturation of the protein followed by 1 (IC₅₀, 28.60±4.10 μg/mL) and 9 (IC₅₀, 35.94±2.10 μg/mL). Diclofenac of sodium (IC₅₀, 7.20±0.97 μg/mL) was used as reference drug

Biography:

Dr. Yogesh V. Ushir has completed his PhD at the age of 30 years from Saurashtra University. He is the Principal of S.M.B.T. Institute of D. Pharmacy, a part of leading SMBT Health organization. He has published more than 30 papers in reputed journals and filed 1 patent in India. He has won bronze medal in foreign scientist category at HTM 2017 conference, held at Bangkok, Thailand in January 2017.

Abstract:

Cecidological and pharmacognostical parameters for the galls of three Ficus species were studied with the aim of drawing the standards. The cecidology, macroscopy, microscopy, powder characteristics, physical standards, thin layer chromatographic studies of Ficus racemosa, Ficus benghalensis, and Ficus religiosa galls included in research work. The study also deals with the phytochemical screening of with various extracts. Here non structural total phenolic content (TPC) determined by Folin- Ciocalteu reagent and flavonoid content (TFC) was determined by aluminum chloride colorimetric method. In addition, total tannin content (TTC) determined by back titration with potassium permanganate also carried out. During the cecidologic study it has been observed that, Ficus species galls have three stages; development, maturation and senescence stage. Microscopically the galls show variation in their growing stages. Commonly cuticle, lignified cells, cortex, starch grains, nutritive tissue and larval chamber were identified during microscopy of galls. The results showed that the moderate presence of terpenes, flavonoid, steroids, phenols and tannins. In study TPC, TFC and TTC are significant and prove that, galls are rich in estimated phytoconstituents and may have pharmacological importance. The cecidological and pharmacognostical standardization studies have been reported for the Ficus racemosa, Ficus benghalensis, and Ficus religiosa galls first time together. 

Biography:

Pargol Mashati has completed  her MSc at the age of  24 years in Hematology and Blood Banking from Shahid Beheshti University of Medical Sciences. She works in the central research laboratory of School of Allied Medical Sciences, Shahid Beheshti University. She has 7 published papers in the field of hematology and  has been researching the combination effect of chemotherapy drugs and natural compounds on cancer cells.

Abstract:

Purpose: The aim of the present study is to investigate the synergistic effect of methanolic extract from aerial parts of Artemisia annua in combination with VCR on leukemic cell death in vitro.

Methods: MTT assay was carried out to determine the cytotoxicity activity of the extract and VCR. Combination Index was evaluated to assess the synergism/antagonism effect of two agents. Apoptosis was analyzed by flow cytometry and Caspase 3 activity assay. mRNA expressions of Caspase 3, Bax, Bcl-2 were quantified using RT-PCR. One-way ANOVA and post hoc Tukey multiple comparison tests were used for statistical analysis.

 Results: Our results showed that the combination of Artemisia annua and VCR caused time and dose-dependent decrease in cell viability and induced apoptosis in both cell lines. Lower doses of VCR and the extract displayed significant synergistic inhibition of Nalm-6 and Reh cell growth (P< 0.001). Interestingly, concurrent use of A. annua and VCR resulted in Caspase 3 and Bax gene upregulation and Bcl-2 gene downregulation (P< 0.05).

Conclusion: From the results presented here, the extract of Artemisia annua substantially increased sensitivity of leukemic cells to VCR, suggesting that this combination could potentially decrease VCR drug side effects.

Biography:

Samavia Mubeen is doing her PhD from Sun Yat-sen University Guangzhou China. She is working on Taxus plant which is popular due to its chemotherapeutic agent taxol. She has published 4 papers in reputed international journals.

Abstract:

Paclitaxel (Taxol) is a most promising chemotherapeutic agent was initially isolated from the inner bark of Taxus brevifolia (Pacific yew). This microtubule stabilizing compound approved by Food and Drug Administration (FDA) against ovarian, breast and lungs cancers. The increase of demand for the drug put the problem of the supply shortage, due to the accumulation of taxol at very low concentration, slow growth of tree and high cost of extraction. For these reasons, alternative sources for taxol production are being sought including the screening of plants other than taxus, chemical synthesis, semi-synthesis by conversion of baccatin III (BAC III) or 10-deacethylbaccatin III (10-DAB III) and microbial fermentation. High cost of production, lengthy steps, low production and difficulty in the extraction process are the main problems encountered for commercial production of taxol from these sources. The most attractive approach for sustainable production of taxol and related taxanes at commercial level is provided by plant cell culture technology. Different strategies such as cell line selection, growth condition optimization, addition of precursors and growth regulators, elicitation, immobilization, biotransformation, two-phase culture system, in situ product removal, and industrial scale-up have been extensively investigated to improve taxol production. This review will shed light on recent advances in taxus cell culture technology, focusing on optimizing strategies to improve taxol and related taxoid production in cell culture system. We will also summarize the progress towards the problem associated with the production of taxol by cell culture technology at the commercial level and will also highlight the recent success.

Biography:

Rizki Dwayana Putra is a student in Faculty of Medicine, Andalas University, Padang. He is interested in diabetes disease and it’s theraphy. He finds in his city that many people diagnosed with type II diabetes are using medical plant (Vernonia amygdalina) to cure themselves. He is interested to do resarch about this plant. 

Abstract:

Statement of the Problem: Diabetes mellitus (DM) is a metabolic disease that occurs due to abnormalities in insulin secretion and/or action. African tea leaf (Vernonia amygdalina; VA) extract had been investigated considered for diabetes therapy due to it’s ability for helping pancreatic cells to synthesize insulin, as well as protecting them from degenerative and oxydative processes.The purpose of this study was to determine the effect of VA extract for decreasing blood glucose levels in alloxan-induced diabetic mice (Mus musculus). Methodology & Theoretical Orientation: We used 15 male albino Swiss Webster mice, weighing 20-30 grams, which were divided into three groups: control group (K1), intraperitoneal single dose alloxan induction (150mg/kg bw) with (P) and without (K2) Vernonia amygdalina 96% alcohol extract supplementation (200mg/kg bw). Alloxan was induced to K2 and P in first day and tested after five days. Vernonia amygdalina extract was admistered to P for next ten days twice a day and was provided before fasting. Fasting glucose was measured twice using rapid glucose test. We used one-way ANOVA and post-hoc LSD test with p<0.05 for between-group comparisons.Findings: We observed an increase in average fasting blood glucose levels in K2 and P after alloxan induction:152.20±15.30 mg/dl and 175.00±44.44 mg/dl, respectively, compared to K1 (80.60±23.38). Glucose levels were significantly different both in K1 to K2 and K1 to P (p<0.05), while not significantly different between K2 and P. After the VA extract administration, the average fasting blood glucose levels in P were significantly decreased (100.60±6.15 mg/dl) compared to K2 (435.80±139.82mg/dl) (p<0.05). Glucose levels were significantly different between K1 (76.60± 8.30 mg/dl) and K2 (435.80±139.82mg/dl), while not significantly different between P and K1. Conclusion & Significance: VA extract showed a favourable effect on glucose metabolism in mice alloxan-induced diabetes model.

Biography:

Ms. Anu Joshi Shrestha currently working as value chain and enterprise development specialist in International Centre for Integrated Mountain Development (ICIMOD) an intergovernmental organization working for 8 countries in Hindu Kush Himalayan region based in Kathmandu. She bring experience of more than 20 years  working with development agencies and bi-lateral/multilateral organizations focusing on high value product value chains, emphasizing the equitable promotion of pro-poor value chains, market development, rural enterprise development and strengthening rural-urban linkages. She was also a faculty member in Kathmandu College of Management, Kathmandu University. She has an MBA from the University of Wales, UK, specializing in marketing and human resources development.

Abstract:

Doing business in mountains has to face mountain specific challenges such as accessibility marginality, fragility and diversity. However mountain have high value niche natural products which opens opportunities for mountain people. But due to its remoteness, limited infrastructure, sparse diverse population, heavy transaction cost makes it impossible for private sectors to look for business in such remotest part in the mountains. There is always the miss match between demand and supply as even though there is a demand for various mountain natural products, the logistical and transactional cost makes the business seem unviable for the private sectors trying to engage in the mountains. Therefore in most cases communities are always in the bottom of the pyramid selling the raw materials receiving minimal value share in the chain. This article tries to bring solution to the demand and supply gap of the mountain through social business enterprise implementing an innovative concept of community business enterprise model to successfully bring out high value products to the niche markets which otherwise is not catered by profit driven conventional manufacturing companies. A social business enterprise is upcoming concept where the private sector which has social orientation along with profit  focus on three bottom line profit, people and planet. Its innovative business model revolves around establishment of community owned rural business enterprises which utilizes local natural resources and value creation using indigenous skills of local population supported by simple technologies.

Biography:

Dr. Gajanand Engla is presently working at School of Pharmacy, Devi Ahilya University Indore, INDIA in the capacity of Assistant Professor; he did his masters in Pharmaceutical biotechnology and has expertise in the field of plant biotechnology specially plant tissue culture techniques and  genetic modifications base bioproduction of secondary metabolites/plant actives. Dr. Engla did his doctoral in the NDDS and the key research area is polymer based drug delivery systems. He has authored three books and about 25 Research papers; presented about 20 papers in seminar/conferences and delivered 05invited talks key note speaker in workshops/symposia/guest lectures

Abstract:

Biography:

Abstract:

Eichhornia crassipes (Mart) Solms commonly known as Water hyacinth belonging to the family Pontideraceae is a free floating warm water aquatic plant native of Brazil. It is an invasive aquatic weed causing serious threats to water ecosystems throughout the world and one of the world’s most obnoxious water weeds when not controlled. Kurichi kulam in Coimbatore is one of the major water bodies that enhances the ground water level around this area. It is often polluted due to invasion of this weed. The Coimbatore city municipal corporation made efforts to remove them periodically with considerable budget expenditure and hence its commercial aspects may be explored. E. crassipes is endowed with many potent phytochemicals like flavonoids, tannins, terpenoids, saponins, cardiac glycosides, quionones and many others. A large number of biologically active compounds have been extracted from this weed. E. crassipes is also considered as a promising alternate renewable source of shikimic acid, which is a critical ingredient used in the formulation of drug oseltamivir commercially known as Tamiflu®, an efficient inhibitor of the human influenza virus H1N1 of swine origin, H5N1 virus causing avian flu. Tamiflu is the only orally administered approved drug for treatment of influenza. Shikimic acid (SA) is mainly sourced from fruits of Chinese star anise (Illicium verum), native to China the lone supplier, but the source is not enough to meet the worldwide demand of shikimic acid, therefore, an alternate source is warranted in order to meet the worldwide demand of shikimic acid and an efficient new technologies needs to be developed for the enhancement of SA production from available sources. Interestingly, shikimic acid is detected in the leaves of E. crassipes which can be harvested in plenty and economically attractive, unlike the star anise that requires six years to harvest the fruits. Therefore, an attempt has been made for the search of shikimic acid from invasive alien weed, E. crassipes as an alternate renewable source of shikimic acid. It is estimated as 3.25% of shikimic acid in leaves of E. crassipes when compared to the Chinese star anise pods (1.77%) which is also higher than many of the reported plant species. Further this content can be enhanced by treating water hyacinth with glyphosate which is a cheap and easily available herbicide. Glyphosate acts as a competitive inhibitor of PEP and binds more tightly to the EPSP synthase-S3P complex where the enzyme is essentially inactivated. Deregulating the shikimate pathway results in the accumulation of very high levels of shikimate and hence application of glyphosate, considerably enhanced the shikimic acid production within a week. HPLC analysis of the extract evidenced that the leaves of E. crassipes contain higher shikimic acid concentrations (3.25%). It is proved from our study that GC-MS-MS analysis revealed the presence of secondary metabolites such as Goniothalamicin, Arborescidine 1, 8 Dipropoxyanthraquinone, Ambrosial Muricin, Camarolide, which account for their promising antioxidant and anticancer activities. The radical scavenging activity of the leaf extract of E. crassipes was found to be 24%. Anticancer activity was studied against human cervical cancer cell line (HeLa) and it showed mild anticancer activity on human cervical 200 μg/Ml. IC50 value was found to be 32.33 μg/mLby MTT assay against HeLa cell lines. cancer cell lines, HeLa with 17% growth inhibition at Therefore it is evident from the present study that commercial utilisation of E. crassipes could be an alternative for the management of the weed to overcome environmental and economic problems posed by it and can potentially be used to meet the emerging needs of both the domestic and the international market.